Major Groove Substituents and Polymerase Recognition of a Class of Predominantly Hydrophobic Unnatural Base Pairs
نویسندگان
چکیده
منابع مشابه
Substituent effects on the pairing and polymerase recognition of simple unnatural base pairs
As part of an effort to develop stable and replicable unnatural base pairs, we have evaluated a large number of unnatural nucleotides with predominantly hydrophobic nucleobases. Despite its limited aromatic surface area, a nucleobase analog scaffold that has emerged as being especially promising is the simple phenyl ring. Modifications of this scaffold with methyl and fluoro groups have been sh...
متن کاملReply to Hettinger: Hydrophobic unnatural base pairs and the expansion of the genetic alphabet.
We have recently reported the successful creation of a semisynthetic organism that has an expanded genetic alphabet by virtue of the retention on a plasmid of either the dNaM-d5SICS or dNaM-dTPT3 unnatural base pair (UBP), the latter of which can be retained at natural-like levels with the use of a Cas9 editing system (1, 2). Despite these demonstrations that a cell can retain a UBP in its DNA,...
متن کاملChapter 9 Minor Groove Recognition of T , A Base Pairs by Benzothiophene / Pyrrole Pairs
Polyamides are designed oligomers that can bind to DNA in a sequence-specific manner with affinities comparable to those of natural DNA-binding proteins. Recently, an N-terminal 3-chloro-6-fluorobenzothiophene polyamide bound in the minor groove of DNA was co-crystallized at 1.1 Å resolution as an antiparallel dimer. In addition, antimicrobial screenings for this polyamide family and the develo...
متن کاملfaculty of psychology and social sciences group of anthropology master thesis in major of anthropology
چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...
KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results fro...
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ژورنال
عنوان ژورنال: Chemistry - A European Journal
سال: 2011
ISSN: 0947-6539
DOI: 10.1002/chem.201102066